Transcriptional profiling of Bmal1WT and Bmal1KO Tregs

We isolated visceral adipose tissue (VAT) Tregs from Foxp3.YFP-Cre Bmal1WT or Foxp3.YFP-Cre bmal1flox mice fed a normal lean diet or a high-fat diet. VAT Tregs were also sorted after adoptive transfer. We found that Bmal1KO Tregs are more activated in lean mice, after 4 weeks HFD and after adoptive transfer, but loseVAT Treg signature after 16 weeks of high-fat diet feeding. Overall design: Foxp3YFP+ Tregs were double-sorted from the visceral adipose tissue of 18-to-30-week-old male at ZT0. For high-fat diet experiments, mice were fed a lean diet for 14 weeks followed by high fat diet of indicated length. For adoptive transfer experiments, Foxp3YFP+ Tregs were double-sorted 9 weeks after adoptive transfer. For the adoptive transfer, the mice were 20 weeks old when they were sacrificed.