Human AML1-ETO driven acute myeloid leukemia (AML) shows a dysfunctional immune microenvironment.

AML is a genetically heterogeneous, aggressive hematological malignancy induced by distinct oncogenic driver mutations. Cancer immunosurveillance is a coordinated response from innate and adaptive immune cells against cancer cells. The effect of specific AML oncogenes on immune activation or suppression has not been investigated. Bone marrow from AML patients or healhy controls were obtained and Ficoll gradient seperated to extract mononuclear cells. Cells were sorted for immune cell fraction (CD34-CD45+) and subject to 3' single cell RNA sequencing using 10x Genomics platform.