Expression analysis of primary mouse mebryonic fibroblasts lacking the POLE4 and P53 tumour suppressors genes

Mouse embryonic fibroblasts lacking the POLE4 subunit of Pol Epsilon show signs of replication stress and DNA damage. Strikingly this phenotype is lost upon deletion of the Trp53 gene. In order to reveal the mechanism responsible for this phenotype we carried oud bulk RNA sequencing from primary mouse embryonic fibroblast WT or KO for POLE4, in a p53 knock-out genetic background or in the presence of one of 2 copies of the wt allele Overall design: Passage 1 primary mouse embryonic fibroblasts from POLE4 wt or ko (null) embryos, wt, heterozygous (het) or knock-out (null) for Trp53 were cultived in low oxygen concentration. Cell were lysed and total RNA was extracted, according to standard procedures.