Analysis of megakaryocyte maturation markers.

*: p<0,05 vs C0; *: p<0,05 vs C0; *: p<0,05 vs C0; *: p<0,0001 vs C0. **: p<0,0001 vs C0; **: p<0,001 vs C0; **: p<0,0001 vs C0; °: p<0,0001 vs C4. °: p<0,0001 vs C4; °: p<0,05 vs C4; °: p<0,05 vs C4; ̂: p<0,0001 vs C8. ̂: p<0,0001 vs C8; ̂: p<0,01 vs C8; ̂: p<0,0001 vs C8; §: p<0,001 vs T4. MEG-01 cells were analysed for their maturation stage after four (T4) and eight (T8) days of treatment with 16 nM TPA and compared with control cells at the same incubation time (C0, C4, C8). Markers of early myeloid (CD34) and mature megakaryocyte stage (CD41, CD42b) were evaluated by flow cytometry analysis, whereas the expression of COX-1 was assessed by western blotting analysis and quantification of immunoreactive bands normalized as a ratio to GAPDH expression. Data from three independent experiments were used and are shown as mean ± S.D. Significance of data referred to treated vs control cells is indicated at the bottom.