Stump project-- mRNA basal layer keratinocytes; Volar versus non-volar acral skin gene expression. Homo sapiens

We were interested in defining the gene signature of volar skin. Overall design: Punch biopsies of skin were split into epidermis and dermis after dispase treatment. Epidermis was trypsinized and sorted for alpha 6 integrin positive basal layer keratinocytes We collected RNA from basal layer keratinocytes of soles and backs of feet and submitted for Affymetrix Exon arrays. 2 replicates of each site from distinct human donors were included; total of 4 samples analyzed