Bacterial Reverse Mutation Assay with and without metabolic activation.

S. Typhimurium strains TA98, TA100, TA1535, TA1537 and E. Coli strain WP2 were incubated with increasing concentrations of test article with or without activation, as indicated. PA and 2-BP were dissolved in DMSO at 100 mg/ml and added to each plate at the indicated concentration for overnight incubation. Reverse mutations were selected and quantified on histidine negative plates. Vehicle negative control and positive control are indicated at the top of the table. Representative of two separate experiments, all data are mean +/− SD. The positive control for strain TA98 with and without metabolic activation was 2-nitrofluorene (1 μg/plate) and 2-aminoanthracene (1 μg/plate), respectively. The positive control for strain TA100 with and without metabolic activation was sodium azide (1 μg/plate) and 2-aminoanthracene (1 μg/plate), respectively. The positive control for strain TA1535 with and without metabolic activation was sodium azide (1 μg/plate) and 2-aminoanthracene (1 μg/plate), respectively. The positive control for strain TA1537 with and without metabolic activation was 9-aminoacridine (75 μg/plate) and 2-aminoanthracene (1 μg/plate), respectively. The positive control for strain WP2 with and without metabolic activation was methyl methanesulfonate (1000 μg/plate) and 2-aminoanthracene (10 μg/plate), respectively.