Comparative analysis of gene expression between overexpression and deficiency of signal peptide peptidase in Arabidopsis thaliana. Comparative analysis of gene expression between overexpression and deficiency of signal peptide peptidase in Arabidopsis thaliana

Signal peptide peptidase (SPP) plays an essential role in among all eukaryotes. The knock out lines of A. thaliana SPP (AtSPP) is lethal and the substrates of AtSPP are not identified. Based on the situation, we constructed the plants with AtSPP over-expressed and knock down to investigate the role of AtSPP. We also used the data to find the candidate substrates of AtSPP, which regulates intermembrane proteolysis, would have crucial physiological functions as well as control the gene expression encoding the substrates. Overall design: Wild type (Col-0) and AtSPP mutants were grown on a half strength of MS medium containing 0.8% agar. Seven days seedlings were collected and subjected to the analysis.