Transcriptional profiling of Ngn3-dependent pancreatic endocrine differentiation at E15.5

This experiment used RNA-Seq technology to explore gene expression in mouse Ngn3^GFP/+ [het] FACS sorted pancreatic cells at E15.5 (committed endocrine progenitor cells) and in Ngn3^GFP/GFP [null] at E15.5 (defective endocrine progenitor cells). This experiment is designed to understand the gene expression alteration in the endocrine lineage at different embryonic days. The aim is to understand both Ngn3 dependent and independent gene expression profiles so as to reveal the instructive signals that specify the collective endocrine islet cell fate or specific islet cell type.本实验采用RNA测序（RNA-Seq）技术，分析两类小鼠胰腺细胞的基因表达特征：一类为胚胎期15.5天（E15.5）经荧光激活细胞分选（FACS）获得的神经源性分化因子3（Ngn3）杂合型（het，Ngn3^GFP/+）已定型内分泌祖细胞，另一类为同胎龄Ngn3纯合缺失型（null，Ngn3^GFP/GFP）缺陷型内分泌祖细胞。本实验旨在解析不同胚胎发育阶段内分泌谱系的基因表达变化规律，最终目标是阐明依赖Ngn3与非依赖Ngn3的基因表达谱，以揭示指导集体性胰岛细胞命运定向或特定胰岛细胞亚型特化的调控信号。