RNA Sequencing Analysis of Nicol-Het, Nicol-KO and Ros1-KO Caput Epididymal Transcriptomes.

Purpose: The goal of this study is to detect differentially expressed genes, between Nicol +/- (Nicol-Het), Nicol-/- (Nicol-KO), and Ros1-/- (Ros1-KO) caput epididymis by RNA sequencing Methods: Caput epidiymal mRNA profiles of 14-week-old Nicol-Het, Nicol-KO, and Ros1-KO mice were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes were downregulated in Nicol-KO caput epididymis compared with Nicol-Het one. The gene downregulation of Nicol-KO caput epididymis was similar to that of Ros1-KO one. Caput epididymal mRNA profiles of 14-week old Nicol-Het, Nicol-KO, and Ros1-KO mice were generated by deep sequencing, in quadruplicate, using Illumina NovaSeq6000.研究目的：本研究旨在通过RNA测序（RNA sequencing），鉴定Nicol杂合型（Nicol +/-，Nicol-Het）、Nicol敲除型（Nicol-/-，Nicol-KO）与Ros1敲除型（Ros1-/-，Ros1-KO）小鼠附睾头组织间的差异表达基因。 研究方法：采集14周龄的Nicol杂合型、Nicol敲除型及Ros1敲除型小鼠的附睾头组织，采用Illumina NovaSeq6000平台进行三次生物学重复的深度测序，以获取各组小鼠的附睾头mRNA表达谱。 研究结果：RNA测序数据成功鉴定出差异表达转录本。 研究结论：相较于Nicol杂合型小鼠，Nicol敲除型小鼠的附睾头组织中多数基因呈现表达下调趋势；且Nicol敲除型小鼠附睾头的基因下调模式与Ros1敲除型小鼠高度相似。本研究同样采用Illumina NovaSeq6000平台，对14周龄的Nicol杂合型、Nicol敲除型及Ros1敲除型小鼠的附睾头组织进行四次生物学重复的深度测序，以获取其mRNA表达谱。