Plaque-associated lipids dataset

This dataset contains MALDI imaging and fluorescence imaging data of 5xFAD mice and control animals. Processed data are provided at either MATLAB arrays or Bruker .slx / .sbd files compatible with SCiLS Lab.

- Animal_1_5xFAD_s1 and s2 : A MATLAB file of 50 micron spatial resolution imaging of whole brain slice from a 5xFAD animal.
- Slide28_Animal1_stitch_channels__Thioflavin S : A PNG file of the corresponding Thioflavin S- stained fluorescence image obtained post-MSI from the same section.
- Slide28_Animal1_stitch_merged : A PNG file of the corresponding merged imaged including brightfield, Thioflavin S (GFP channel) and Hoechst staining (DAPI channel) used for image registration
- mz_bins_use_neg.mat : A MATLAB array of the m/z channels all MSI images (whole brain slice, 50 micron spatial resolution) were binned to in order to enable comparison
- Animal3_S18_HR.mat : A MATLAB array of high-spatial-resolution (5 micron) imaging of a 5xFAD mouse hippocampus and cortex. Due to the large dataset, 22 m/z channels are included.
- Animal5_S17_HR.mat : A MATLAB array of high-spatial-resolution (5 micron) imaging of a wildtype mouse hippocampus and cortex
- mz_features_22.mat : A MATLAB array of the 22 m/z channels included in the high spatial resolution imaging data
- MALDI_Animal03_5xFAD_10um_neg.zip : A folder containing .slx and .sbd MALDI imaging data of a 5xFAD mouse (whole-brain section) at a 10 micron pitch (used in Figure 5)
- MALDI_animal3_5xFAD_5um_neg.zip : A folder containing .slx and .sbd MALDI imaging data of a 5xFAD mouse (region of interest) at a 5
micron pitch (used in Figure 4)
- TIMS_animal1_20um_5xfad_neg.zip : A folder containing .slx and .sbd MALDI TIMS imaging data of a 5xFAD mouse (region of interest) at a 20 micron pitch (used in Figure 7)
- TIMS_animal5_s13_20um_wt_neg.zip : A folder containing .slx and .sbd MALDI TIMS imaging data of a wild typemouse (region of interest) at a 20 micron pitch (used in Figure 7)
- Region Masks BMC.zip : A folder containing masks from brain regions of spatial transcriptomics data from the BMC Genomics data set
- Region Masks Cell.zip : A folder containing masks from brain regions of spatial transcriptomics data from the Cell data set