RNA-seq analysis of metabolic tissues in high salt diet fed mice

The goal of this study is to understand the effects of high salt diet on metabolic changes in different tissues including liver, WAT and muscle through RNA-seq. Methods: Liver, WAT and muscle mRNA profiles of control WT and 12wks high salt diet fed mice were generated by RNA deep sequencing, using Illumina Hiseq 6000. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using Hisat2. Results: We observed that several genes associated with de novo lipogenesis and cholesterol biosynthesis were significantly down-regulated in WAT and liver tissue of HSD mice group. Besides, combined with secretome datasets, our results further demonstrated that high salt diet could also down-regulate different organokines, and thus, possibly mediate crosstalk between different metabolic tissues Conclusions: Our study investigated the mechanisms of changed gene expression pattern of metabolic organs induced by high salt in terms of whole transcriptome profiling. mRNA profiles of liver, WAT, muscle tissues of high salt fed C57BL6J mice本研究旨在通过RNA测序（RNA-seq）探究高盐饮食对肝脏、白色脂肪组织（WAT）及肌肉等不同组织的代谢变化的影响。方法：本研究采用Illumina Hiseq 6000平台，对野生型（WT）对照小鼠及喂食12周高盐饮食的小鼠的肝脏、WAT及肌肉组织的mRNA进行深度测序，获取其转录组表达谱。对测序得到的reads进行接头序列修剪、低复杂度或低质量序列屏蔽，随后使用Hisat2将处理后的reads比对至mm10全基因组。结果：本研究观察到，高盐饮食（HSD）小鼠组的WAT与肝脏组织中，多个与从头脂肪生成及胆固醇生物合成相关的基因均显著下调。此外，结合分泌组数据集进行联合分析，本研究结果进一步证实高盐饮食还可下调多种器官因子（organokines），从而可能介导不同代谢组织间的信号串扰。结论：本研究通过全转录组分析，阐明了高盐饮食诱导的代谢器官基因表达模式改变的机制。本研究生成了高盐饮食喂养的C57BL6J小鼠肝脏、WAT及肌肉组织的mRNA表达谱。