Genomic analysis of multi-drug resistant coagulase-negative staphylococci from healthy humans and animals revealed mobilomes of unusual mechanisms and CRISPR-Cas system

Coagulase-negative staphylococci are evolving as major reservoirs and vectors of unusual and critical antimicrobial resistance (AMR). In this study, the genomic characterization of 26 multidrug resistant-CoNS (resistant to four or more classes of antimicrobial agents) previously obtained from the nasal cavity of nestling storks, healthy humans who had no contact with animals, healthy pigs, healthy pig farmers, healthy dogs and dog-owner from Spain, were performed by Illumina technology. High-quality draft genomes were used to determine their resistome, virulome, mobile genetic elements and CRISPR-Cas types. The relatedness of three CoNS species with publicly available genomes was assessed by core-genome single nucleotide polymorphisms .