Global crop introduction drives host jumps, turning native Pathogens into emerging diseases

This Dryad submission contains the primary datasets, metadata, and analytical outputs underlying the conclusions of Crouch et al. 2026 (PNAS). The dataset includes multilocus DNA sequence alignments (ITS, LSU, and additional loci as analyzed), specimen-level metadata for fresh and herbarium-derived samples (including host, geographic origin, collection date, and voucher information), geographical figures, and single loci phylogenetic tree. Population genetics data has been submitted to T-BAS (https://guide-tbas.cifr.ncsu.edu/tbas) and all sequence files have been submitted to GenBank (https://www.ncbi.nlm.nih.gov/genbank/). 
Data values consist of nucleotide sequence data (FASTA and alignment formats), geographic locality descriptors, and host association data. Herbarium-derived sequence data include century-old specimens. All specimen identifiers are cross-referenced with voucher information to facilitate reproducibility and reuse.
These data support reconstruction of geographical..., Detailed laboratory and analytical methods are provided in the Supporting Information of Crouch et al. (2026, PNAS). Briefly, DNA was extracted using a Chelex protocol and six loci (ITS, 28S, GAPDH, IGS, RPB2, TUB) were amplified and sequenced. Multilocus concatenated alignments were used for phylogenetic inference. Haplotype networks were inferred using TCS, split networks were constructed using SplitsTree, and isolation-with-migration analyses were performed in IMa3.
Full methodological details are available in the Supporting Information of Crouch et al. (2026, PNAS).
, # Data from: Global crop introduction drives host jumps, turning native Pathogens into emerging diseases

Dataset DOI: [10.5061/dryad.ht76hdrxf](https://doi.org/10.5061/dryad.ht76hdrxf)

## Description of the data and file structure

This dataset contains sequence data, phylogenetic trees, specimen metadata, and figure source files used to generate analyses and figures in:

Crouch et al. (2026), *Proceedings of the National Academy of Sciences*.

The dataset supports multilocus phylogenetic reconstruction of powdery mildews infecting *Fragaria* (strawberry) and *Rubus* (raspberry), including historical herbarium specimens and contemporary collections. Data include individual locus FASTA files, Single Locus Bayesian phylogenetic trees, geographic distribution mapping, and specimen-level metadata.

All nucleotide sequences are deposited in GenBank and are provided here as FASTA files to facilitate reuse.

### Files and variables

#### **Files Included**

**1. Sequence Data (FASTA format)*..., ,