Bacterial and fungal communties in co-located disease suppressive and conducive soils

Disease-suppressive soils are exceptional ecosystems supporting plant health even in the presence of pathogens, and microbial mechanisms involved in pathogen suppression have been deciphered in various crops. However, it remains unclear whether multiple located disease-suppressive soils against same soil borne disease harbor general and coincident mechanisms. To disentangle the underlying mechanism, amplicon sequencing of bulk soil microbiome from six pair-located banana Fusarium wilt disease suppressive or conducive orchards were performed and results showed that disease suppressive soils revealed distinguish core but not whole bacterial community composition and harbored shared key bacterial taxa involved inthedisease suppression, among which Pseudomonas was inferential as a key biocontrol agent in core bacterial community linking to pathogen suppression. Subsequent greenhouse experiments testing antagonistic Pseudomonas strains isolated from disease suppressive soils confirmed their positive Fusarium wilt disease suppression effects. Metagenomic analysis further confirmed a high density of Pseudomonas and revealed an up-regulation of functions related to the synthesis of indole-3-acetica, quorum sensing and potent antimicrobial compounds in disease suppressive soil. Together we demonstrated that the action of disease suppressive soils in a small scale that Pseudomonas enriched core microbial consortia were shared and distributed in different locations for suppressing the same soil borne disease.