Effect of the overexpression of the NOTCH inhibitor ligand DLL3 in low grade astrocytoma cell line LGG275

Astrocytoma are known to presents a cellular heterogeneity consisting of three identified subpopulation: "astrocyte-like", "oligodendrocyte-like" and "NPC-like" cells. Among these populations, "astrocyte-like" cells show plasticity and can transit to an "oligodendrocyte-like" state. Activation of NOTCH pathways induce an transition into astrocyte-like state. To investigate the effect of NOTCH inhition on cell plasticity, we overexpressed DLL3, the main ligand inhibitor of NOTCH. Overall design: To investigate the effect of DLL3 overexpression in LGG275 astrocytoma cell lines, we used an lentivirus expressing luciferase (Ctrl) or DLL3 as well as GFP protein. RNA sequencing was realized on cells in presence or absence of growth factors EGF and FGF2.