Algesic MBP peptide induce pain-specific signaling in rodent Schwann Cells

We demonstrated the pain-specific response to the algesic peptide fragment MBP84-104 of myelin basic protein that induces pain if injected into sciatic nerve of rats and mice. We used the wild-type peptide MBP 84-104, H89G mutant peptide (MBP84-104-H89G), scramble peptide (MBP84-104-SCR) and phosphomimetic peptide MBP84-104-mimTT to stimulate the primary rat Schwann cell cultures. After 24h we isolated total RNA and conducted genome wide RNA-seq. In addition, we performed RNA-seq using Schwann cells constitutively expressing an MBP84-104-mCherry construct. The gene expression data was analyzed using Ingenuity Pathway Analysis software. We conclude that the Schwann cells expressing MBP84-104 constructs stimulate pain-specific signaling pathways thus representing a relevant model to study neuropathic pain.本研究证实了髓鞘碱性蛋白（myelin basic protein, MBP）的痛觉肽片段MBP84-104可引发痛特异性应答——该片段注入大鼠与小鼠坐骨神经后可诱发疼痛。本研究采用野生型肽MBP84-104、H89G突变肽（MBP84-104-H89G）、乱序肽（MBP84-104-SCR）以及磷酸化模拟肽MBP84-104-mimTT，对原代大鼠雪旺细胞（Schwann cell）培养物进行刺激。刺激24小时后，我们提取总RNA并开展全基因组RNA测序（RNA-seq）。此外，我们还对组成型表达MBP84-104-mCherry融合构建体的雪旺细胞进行了RNA测序。采用英格纽特通路分析（Ingenuity Pathway Analysis, IPA）软件对基因表达数据进行分析。本研究最终得出结论：表达MBP84-104相关构建体的雪旺细胞可激活痛特异性信号通路，因此该模型可作为研究神经性疼痛的可靠体外模型。