Gene expression profiling of novel glucocorticoids for severe asthma in RAW264.7 cells

To investigate the anti-inflammation activity of novel glucocorticoids, we used microarray to profile gene expression pattern of novel GC VSG158 and VSG159, in parallel with FF and DEX, in the mouse macrophage RAW264.7 cells. RAW264.7 cells with various glucocorticoids (Dexamethasone, VSG158, VSG159, and fluticasone furoate) were treated with designated steroids at 100 nM one hour before overnight LPS stimulation of inflammation. Each treatment was duplicated. The following day, RNA samples were isolated using a Qiagen mini RNA extraction kit for gene profiling.