Massively parallel whole-organism lineage tracing using CRISPR/Cas9 induced genetic scars. Danio rerio

A key goal of developmental biology is to understand how a single cell transforms into a full-grown organism consisting of many cells. Although impressive progress has been made in lineage tracing using imaging approaches, analysis of vertebrate lineage trees has mostly been limited to relatively small subsets of cells. Here we present scar-trace, a strategy for massively parallel whole-organism lineage tracing based on Cas9 induced genetic scars in the zebrafish. Overall design: Sequencing of CRISPR/Cas9 induced genetic scars was done by targeted PCR A Cas9-based method for massive genomic marker-based lineage tracing in zebrafish. Plase note that the 'Barcodes_zf1fin3.xlsx' annotates GSM2258256-GSM2258282 data.