mRNA-seq analysis of Caenorhabditis elegans starved L1 larvae with and without AMA-1/Pol II degradation in the soma and germ line during L1 arrest

Whole starved L1 larvae were collected with and without AMA-1 degradation by the auxin-inducible degron (AID) system in the soma and germ line between 36 and 132 hours of starvation along with controls. Overall design: 4 strains each collected at 3 time points/conditions during L1 starvation: 36 hr, 132 hr with auxin, and 132 hr with ethanol. For auxin samples, auxin was added at 36 hr. 4 biological replicates were collected for each strain and time point (48 samples total).