Paenibacillus sp. Dod16 Genome sequencing

Tsetse flies, the only cyclical vectors of African trypanosomes, responsible for human African trypanosomiasis and animal African trypanosomiasis (Krafsur, 2009) harbour diverse communities of microorganisms, especially bacteria. It composition varies considerably depending both on the tsetse species and the geographic origin of the flies (Geiger et al., 2013). This microbial community influences several aspects of tsetses physiology, including nutrition, fecundity and immune system (Wang et al., 2013). The interactions between tsetse flies and their microbiota are thought to play a critical role in shaping the flies capacity to transmit diseases. Previous studies have explored the microbiota of tsetse flies, identifying symbiotic relationships that may impact disease transmission (Attardo et al., 2020; Ngambia Freitas et al., 2021). However, little research has focused on the role of Paenibacillus species in this context, which is critical for exploring new strategies for controlling trypanosomiasis.Paenibacillus, a genus of rod-shaped, Gram-positive, motile bacteria has been detected in a variety of environments, such as: soil, water, rhizosphere, vegetable matter, forage and insect larvae, as well as clinical samples. Paenibacillus produces a diversity of antimicrobials, enzymes, and exopolysaccharides with relevance in medicine, process manufacturing, and bioremediation, some of which have already been commercialized (Grady et al., 2016).Despite previous studies on tsetse microbiota, little is known about the potential role of Paenibacillus species in trypanosomiasis control. This study seeks to bridge this gap by exploring the genetic features of the Paenibacillus species isolate Dod16. The bacterium was isolated from the gut of tsetse fly caught in Dodeo, Cameroon. The whole genome was sequenced on the Illumina Miseq platform. Sequences were assembled with SPAdes and annotated using RAST annotation pipelines.