Kuo-2014-Genetics-Fig3

These primary data are summarized and discussed in Kuo et al., Genetics (2014). Data in this file are from the P{wa} assay, first published in Adams et al., Science (2002), DOI: 10.1126/science.1077198 . The first tab has counts of progeny from each vial. As in other uses of this assay, only daughters that did not recieve the transposase chromosome (Cy+) were scored. Since the Df(3R)ED6058 chromosome has w+ on it, only progeny that recieved a Sbchromosome were scored. f red = frequency of red-eyed flies f yellow = frequency of yellow-eyed flies The last tab has molecular analysis of synthesis and/or deletion in the yellow-eyed daughters. Each row represents presence (+) or absence (-) of a PCR product in white-eyed sons of yellow-eyed progeny. Each yellow-eyed progeny came from a different vials (f numbers are Fancm, c numbers are control). Distance of furthers primer from left or right end of the element is indicated. Primers used are listed.