Identification of ParB binding sites in vivo in Pseudomonas aeruginosa. Pseudomonas aeruginosa

To identify ParB binding sites in vivo, we performed ChIP experiments followed by high throughput sequencing using a 3xflag tagged version of ParB, in different genetic backgrounds. This allowed us to show that ParB binds in vivo mostly to 4 parS sites, located within 15 kb of oriC. We also identify 9 secondary ParB binding sites. Overall design: A functional ParB-3xflag was used for ChIP-seq experiments.