Genome-wide gene expression analysis for CRISPRi targeting a human SFTPB locus bound by NKX2-1

RNA-seq was performed to identify genes affected by CRISPRi (dCas9-KRAB) with sgRNA (sgSFTPB#2) targeting the first intronic region of human SFTPB bound by NKX2-1 in an unbiased genome-wide fashion using A549 lung carcinoma cells that constitutively express dCas9-KRAB and NKX2-1. Expression of genes in A549 cells stably expressing dCas9-KRAB and NKX2-1 with the sgRNA targeting the SFTPB locus was compared with those stably expressing dCas9-KRAB and NKX2-1 with a sgRNA of non-targeting negative control.