Quantification of correlation between MyoG or MyHC positive cells and MitoTracker positive cells.

Myotubes during differentiation were stained with MitoTracker and Hoechst and MyoG or MyHC. The total numbers of nuclei in MyoG-positive, MyHC-positive, and MitoTracker-positive cells were counted using each fluorescence micrographs (n = 12). The total numbers of nuclei in double (“MyoG and MitoTracker” or “MyHC and MitoTracker”) positive cells were also counted using merged micrographs (n = 12). The percentage of total number of nuclei in each double positive cell over the total number of the nuclei in indicated single positive cells was calculated.