Quantification of correlation between MyoG or MyHC positive cells and MitoTracker positive cells.
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Myotubes during differentiation were stained with MitoTracker and Hoechst and MyoG or MyHC. The total numbers of nuclei in MyoG-positive, MyHC-positive, and MitoTracker-positive cells were counted using each fluorescence micrographs (n = 12). The total numbers of nuclei in double (“MyoG and MitoTracker” or “MyHC and MitoTracker”) positive cells were also counted using merged micrographs (n = 12). The percentage of total number of nuclei in each double positive cell over the total number of the nuclei in indicated single positive cells was calculated.
创建时间:
2015-12-02



