Single-nucleus RNA sequencing reveals cellular transcriptome features at different growth stages of porcine skeletal muscle

Porcine latissimus dorsi muscle is one of the main sources of pork products. Meat quality indicators such as the proportion of muscle fibres within the latissimus dorsi muscle and intramuscular fat deposition change during the growth and development of pigs.We used single-nucleus sequencing (snRNA-seq) to analyse the changes occurring in the longest dorsal muscles of pigs at different growth stages Skeletal muscle tissue was obtained from the latissimus dorsi muscle of 170-day-old and 245-day-old white pigs, respectively, for single nucleus sequencing.