Circular RNA YPEL2 promotes cell proliferation, migration and invasion as a biomaker in cervical cancer

Circular RNA (circRNA) is newly type of noncoding RNA that are formed by back-splicing of exon and play the important functions in tumorigenesis. However, the research of circRNA is deficient in cervical cancer (CC). We performed high-throughput sequencing on cervical cancer tissues and normal cervical control samples. We discovered the up-regulated circYPEL2 (has_circ_0005400) in cervical cancer and used this as a potential research object through bioinformatics analysis. Finally, through RNA sequencing and bioinformatics technology, we analyzed the downstream targeted genes that may be affected by circYPEL2. Cervical cancer tissues and normal cervical control samples were collected from 3 cervical cancer patients and 3 normal patients. Total RNA was extracted from these samples, and the RNA was reverse transcribed to cDNA and constructed into a strand-specific library. Illumina HiSeq2000 was performed for sequencing. We designed short interfering RNAs (si-NC, si-circYPEL2-1, si-circYPEL2-2) to transfect into HeLa and SiHa cell lines. After verifying by qRT-PCR, we found the si-circYPEL2-2 is the most efficient. So we used it for three independent replicates to knock down circYPEL2. Total RNA was extracted, and the RNA was reverse transcribed to cDNA and constructed into a strand-specific library. Illumina HiSeq2000 was performed for sequencing.