Dataset for calculating lattice vectors (and anisotropy) of zebrafish cone mosaic

Tg(sws1:nEos) fish. Juvenile zebrafish (0.7 to 0.88 cm standard body length) were anesthetized with 0.672 mg/ml Tricaine S/ MS-222 (Western Chemical Inc., Ferndale, WA) and placed dorsal side down on a 50 mm glass bottom petri dish with a No. 1.5 coverslip (MarTek Corporation, Ashlan MA) and held in place with damped Kimwipes. Imaging and photoconversion were performed with a Leica TCS SP8 LSCM (Leica Microsystems, Werzlar, Germany) equipped with Leica 40X PL APO CS2 Water Immersion lens, 1.1 NA with 650 um working distance. Green to red photoconversion of nEOS protein was performed by a 405 Diode laser at 400 Hz scan speed with a resolution of 512 x 512 pixels in the xy dimension at a single optical plane. Pre and post photoconversion images were captured with the White Light Laser tuned to 506 nm for nEOS (green) and 573 nm for nEOS (red). Leica HyD hybrid detectors were tuned to 516-525 nm for nEOS (green) and 620-761 nm nEOS (red).