FcRL3 expression defines specialized cytotoxic human memory T lymphocytes

To characterize the FcRL3+ T cell subset in memory helper CD4+, CD8+, and CD4+ regulatory lymphocytes, we performed RNA-sequencing analysis using T cells, separated by the expression of FcRL3, from the peripheral blood of 5 healthy donors, stimulated for 3h with PMA and ionomycin to induce cytokine expression. Memory helper CD4+, CD8+ and CD4+ regulatory T cells from 5 independent donors were sorted by the expression of FcRL3. Total RNA was extracted from each sample, used for poly-A library preparation following the SMART-seq2 protocol and sequenced with Illumina NOVASeq 6000 platform.