GNPS - Symbiotic squid minimum biomass test NEG

This project is in collaboration with the McFall-Ngai and Ruby labs investigating the effects of symbiosis on the metabolome of the Hawaiian bobtail squid Euprymna scolopes. This data was collected from symbiotic (SYM; Vibrio fischeri ES114 colonized) and aposymbiotic (APO; no symbiont exposure) squid, flash frozen 24 hours post hatch. This analysis a test to determine the minimum required biomass to successfully perform untargeted LC-MS/MS on juvenile squid, as well as establish a baseline metabolome for whole squid (both SYM and APO). Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 150 x 2.1 mm (Phenomenex, Torrance, CA). LC-MS/MS data was acquired in negative mode