Data on hypoxia effects on cellular responses to WAF and CEWAF solutions in sheepshead minnow larvae

This dataset contains the raw data obtained in support of the publication: Dasgupta, S., DiGiulio, R.T., Drollette, B.D., L Plata, D., Brownawell, B.J., & McElroy, A.E. 2016. Hypoxia depresses CYP1A induction and enhances DNA damage, but has minimal effects on antioxidant responses in sheepshead minnow (Cyprinodon variegatus) larvae exposed to dispersed crude oil. Aquat. Toxicol. 177 (250-260). (WAF) or chemically enhanced dispersed crude oil (CEWAF) were made from Southern Louisiana Sweet Crude (Surrogate) Oil. CYP1A activity was measured in single live larvae using the in vivo EROD (ethoxy resorufin O-deethylase) assay. DNA damage was assessed using the Comet Assay on whole larvae. Activities of a suite of oxidative stress enzymes including catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and glutamate cysteine ligase (GCL) were measured, as well as levels of total, reduced and oxidized glutathione (tGSH, GSH, GSSG), and lipid peroxidation, using the thiobarbituric-acid reactive substances (TBARS) assay.