RNA-seq of in vitro derived B cells

IFN-? has been reported to be the ABC-promoting cytokine combined with signaling from IL-21, CD40L, Ag-BCR and TLR7 agonist. Hence, we adopted the in vitro differentiation cocktails (anti-IgM, R848, anti-CD40, IL-21 plus IFN-? or IL-4) to determine the role of IFN-? in ABC differentiation. Mouse splenic B cells were purified by negative selection and cultured in the presence of the above cocktails. Compared with IL-4, IFN-?-polarized cells preferentially differentiated to ABC-like cells. And RNA-seq were performed on IFN-?-polarized cells and IL-4-polarized cells.