Analysis of the transcriptome response to drought in Larix kaempferi using single-molecule long read isoform sequencing with de novo RNA-seq reads

Larix kaempferi is important afforestation species in northeastern China and is one of the most drought-tolerant pine species.Larix kaempferi has a very complex genetic background and a large genome, and study on the molecular mechanisms of drought resistance is still lag behind. Four-month-old of L. kaempferi seedlings grown in the greenhouse into two groups, the drought treatment group was no watering for 14 d (D14), while normally watered seedlings were used as control group (CK). All plants harvested on the same day and at the same time for the upper part of seedling (circa 3 cm from the top), frozen in liquid nitrogen. We designed six samples (CK-1, CK-2, CK-3, D14-1, D14-2, and D14-3) in total for single-molecule long-read sequencing technology to reannotate the Larix kaempferi genome.