Gene expression of fly testes with Med22 knock down. Drosophila melanogaster

The effect of Med22, spermatocyte arrest (sa) and always early (aly) loss of function on the gene expression in spermatocytes was assessed by microarray. Title: Recruitment of Mediator complex by cell type and stage-specific factors required for tissue-specific TAF dependent gene activation in an adult stem cell lineage Overall design: RNA was obtained from whole testes of flies with following mutations: 1)bam-gal4: UAS-Med22RNAi (VDRC#104581). Three biological replicates were included. Experiments were performed at 25C.