Micrographs of strains with MultiduBE before and after induction

For the verification of MultiduBE-promoted fluorescent and morphology diversification, the crRNA array YKTmf5C-Sp4 was assembled into the mixture of pWLT-duBE-1a and pWLT-duBE-2b and then transformed into strain BSZRG-CmYKT. The results colonies were scraped from the plate and inoculated in a 50-mL sterile tube containing 10 mL LB media with kanamycin for pre-culture for about 10 h. Subsequently, the pre-culture was inoculated in a 14-mL sterile tube containing 2 mL LB-kanamycin media without aTC and a 14-mL sterile tube containing 2 mL LB-kanamycin media with 0.5 μM aTC, respectively. The two group cultures (without induction and with induction) were analyzed by Laser Scanning Confocal Microscopy (LSCM) and flow cytometry., ,