Data from: Identification and validation of single nucleotide polymorphisms (SNPs) as tools to detect hybridization and population structure in freshwater stingrays

Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, P. motoro and P. falkneri, using double digest Restriction-site Associated DNA (ddRAD) reads using 454-Roche technology. A total of 226,774 reads (65.5 Mb) were obtained (mean read length 289 ± 183 bp) detecting a total of 5,399 contigs (mean contig length: 396 ± 91 bp). Mining this dataset, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P. falkneri, 21 in P. motoro, 3 in both species, and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.