CRISPResso2 CD33 editing analysis

For CD33 editing analysis, genomic DNA isolated from the FACS sorted T, B, NK and myeloid cells were PCR amplified at the CD33 targeted site, followed by deep sequencing of the amplicons using the MiSeq System (Illumina). Small insertions and deletions (InDels) generated by CRISPR/Cas9 editing for each amplicon were analyzed using a bioinformatics analysis pipeline based on CRISPResso2 and the frequencies of all InDels were combined and reported as percent editing.