Elevation of components of the GLUT4 trafficking pathway during mouse skeletal muscle regeneration.

A) VAMP2 and CHC22 levels during muscle regeneration after cardiotoxin injection on day 0 were compared between WT and CHC22-mice in muscle samples harvested on the day indicated. One typical set of immunoblots is shown at the left and quantification of VAMP2 relative to GAPDH signals from three experiments generated the ratios plotted at the right (upper plot). There was a statistically significant difference between WT and CHC22-mice in VAMP2 expression on day 14 after cardiotoxin injection (*p<0.05), as determined by Student’s t test. Quantification of VAMP2 and CHC22 relative to GAPDH in the CHC22-mice shown at the left was plotted (right, bottom). B) GLUT4 levels during muscle regeneration for wild type and CHC22-mice (n = 3). One typical set of immunoblots is shown at the left and quantification of GLUT4 relative to GAPDH signals is plotted on the right. No significant difference between WT and CHC22-mice was detected. Molecular mass (kilodaltons) of the proteins detected is indicated at the right.