The effect of age on alternative splicing in different tissues of wild-type mice

Exon level expression analysis for the physiological aging study data set to analyze the effect of age on alternative splicing in different tissues and age groups of wild-type mice Analysis of the effect of age on alternative splicing (AS) using exon microarrays to interrogate the differential exon usage of the entire genome of aging wild-type male C57BL/6J mice (4- and 18-month-old) in five tissues (skin, skeletal muscle, bone, thymus, and white adipose tissue) and in an additional 28-month-old age group, which allowed for age-related AS analysis of the skin, skeletal muscle and bone tissues. We found AS genes with age in all tissue, we show that the number of AS genes increased with age and that AS genes across all tissues are involved in RNA processing. Note: This dataset is one of the 2 datasets in the overall study. An additional data set series is available with exon expression analysis of HGPS mice to analyze the effect of progerin expression on alternative splicing. The two datasets are linked together in the SuperSeries GSE67289. A link to the SuperSeries is available at the bottom of this page. 65 tissue samples from wild-type male C57BL/6J mice; from 5 different tissues (ventral skin, skeletal muscle, bone, muscle, and white adipose tissue) and from 3 different age groups: 4, 18 and 28 months (for skin skeletal muscle and bone ) and from 2 different age groups: 4 and 18 months (for ventral skin, skeletal muscle, bone, thymus and white adipose tissue)本数据集为生理衰老研究相关的外显子水平表达分析（exon level expression analysis）数据集，旨在探究年龄对野生型小鼠不同组织、不同年龄组的可变剪接（alternative splicing, AS）的影响。本研究采用外显子微阵列（exon microarray）技术，对4月龄与18月龄的衰老雄性野生型C57BL/6J小鼠的全基因组外显子使用差异进行检测，以此分析年龄对可变剪接的影响；实验覆盖皮肤、骨骼肌、骨骼、胸腺、白色脂肪组织共5种组织，并额外增设28月龄组，由此可针对皮肤、骨骼肌与骨骼组织开展年龄相关可变剪接分析。我们在所有组织中均发现了随年龄表达变化的可变剪接基因，证实可变剪接基因的数量随年龄增长而增加，且所有组织中的可变剪接基因均参与RNA加工过程。注：本数据集为本次整体研究的两套数据集之一。另有一套数据集系列针对哈钦森-吉尔福德早衰症（Hutchinson-Gilford Progeria Syndrome, HGPS）小鼠开展外显子表达分析，用以探究早老素（progerin）表达对可变剪接的影响。两套数据集已通过超级数据集（SuperSeries）GSE67289进行关联，本页面底部提供了该超级数据集的链接。本数据集共包含65份雄性野生型C57BL/6J小鼠组织样本，覆盖腹侧皮肤、骨骼肌、骨骼、肌肉与白色脂肪组织共5种组织，并按两类分组标准采样：针对皮肤、骨骼肌与骨骼组织，设置4、18、28月龄共3个年龄组；针对腹侧皮肤、骨骼肌、骨骼、胸腺与白色脂肪组织，则设置4、18月龄共2个年龄组。