AncMcPK

The work is related to studying the evolution of the allosteric specificity for AMP in the last common ancestor of Pyruvate Kinase (PK) enzymes from methanogenic archaea. This, since there is no X-ray structure of PK from methanogenic archaea, and to obtain structural information of allosteric regulation of this protein can help to understand the glycolytic pathway flux control in methanogenic archaea. We currently have screened cryo-EM grids for this protein obtaining good quality 2D classes from different orientations of the protein. Now we require to collect data of a more concentrated sample, to obtain more particles in the less favored orientation.