Brassica napus Transcriptome or Gene expression

Transcriptome study provides an important framework for examining the relationship between genotype and phenotype, which give better knowledge to understand several pathways and mechanisms related to suggested traits. RNA sequencing used widely to investigate changes in global gene expression, which showed higher sensitivity and effectiveness than microarray analysis. The previous study explained that It is a broad collection of transcripts or RNA profiles found in a cell at a particular physiological or developmental stage.Ovules are the direct precursors of seeds, and ovule number determines seed crop yield and plant fitness. The present study disclosed the physiological processes, genetic markers, and genes governing ovule number variation in rapeseed. Therefore, RNA-sequencing is a key method for dissecting the functions of the genome and identifying the molecular structure of cells and tissue. The two extreme ovule number groups were subjected to transcriptome analysis, and 4449 differentially expressed genes (DEGs) were identified. Differences in ovule number between the extreme lines can be attributed to photosynthesis, transport, hormonal communications, and transcription regulations. Our results offer important resources for in-depth exploration of the complex genetic networks controlling ovule number through RNA-seq.