Uncovering the mechanisms-based quality control of traditional Chinese medicine against essential hypertension by network analysis and experimental validation

A total of 4276 lncRNAs (1426 upregulated and 2850 down-regulated) and 9230 mRNAs (5187 upregulated and 4043 down-regulated) were differently expressed in SHR-C compared with WKY, whereas 265 lncRNAs (65 up-regulated and 200 down-regulated) and 267 mRNAs (132 upregulated and 135 down-regulated) were differently expressed after BSJY treatment (fold change >2. 0 and P value< 0. 05). Taking the intersection of the two sets, the analysis found that 8 lncRNAs and 35 mRNAs were upregulated, whereas 11 lncRNAs and 15 mRNAs were downregulated in the WKY vs. SHR-C group and SHR-Bh group (fold change > 2. 0 and P value < 0. 05). It showed the heatmap that were constructed using fold change, It showed the Volcano plots that were constructed using fold change values and P values and revealed the relationship between fold change and statistical significance.与WKY大鼠相比，SHR-C组共计存在4276个长链非编码RNA（lncRNAs，其中1426个上调、2850个下调）及9230个信使RNA（mRNAs，其中5187个上调、4043个下调）呈现差异表达；经BSJY干预后，仅存在265个lncRNAs（65个上调、200个下调）与267个mRNAs（132个上调、135个下调）呈现差异表达（折叠变化>2.0且P值<0.05）。取上述两组差异基因集的交集进行分析后发现，在WKY与SHR-C组、SHR-Bh组（折叠变化>2.0且P值<0.05）中，共有8个lncRNAs与35个mRNAs呈上调表达，11个lncRNAs与15个mRNAs呈下调表达。本研究构建了基于折叠变化的热图，以及基于折叠变化值与P值的火山图，用以直观展示折叠变化与统计学显著性之间的关联。