Spec-seq data for ZNF140, ZNF10, ZFP3, ZNF675, ZNF382

This dataset consists of Spec-seq samples data for four tandem zinc finger proteins in human genome, each with two replicates. The Spec-seq is a medium throughput technique to characterize the binding specificity of a TF of interest to thousands of binding sites with resolution down to 0.2kT. Similar samples were deposited to NCBI GEO database before (GSE188166). The data analysis workflow for each ZFP can be accessed through https://github.com/zeropin/ZFPCookbook. For ZNF140, a truncated construct containing finger 1 to 6 (F1-F6) was fused to N-terminal Halo tag for protein expression and EMSA assay; For ZNF10, F1-F8 construct was used; For ZFP3, F1-F10 construct was used; For ZNF675, both F1-F11 and F1-F6 constructs were tested separately; The DNA library design and experimental workflow was described in a paper to be published in detail. The Spec-seq libraries for ZNF675 includes not only synthetic elements but also 500 endogeneous genomic elements from human genome (300 sites within repeat elements, 200 sites outside repeat elements). For ZNF382, its DNA-binding domain including 9 fingers was fused to N-terminal HALO-Tag for experimental work. The DNA library design and experimental workflow was described in paper to be published in detail.