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Tuxedo bug report: H. saltator simulated data

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DataCite Commons2020-09-04 更新2024-07-25 收录
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https://figshare.com/articles/dataset/Tuxedo_bug_report_H_saltator_simulated_data/1300104
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I randomly selected a 150kb region from <em>Harpegnathos saltator </em>to create a demo data set for instruction. I extracted sequences for the transcripts encoded in the region and used wgsim to simulate Illumina reads for these transcripts. The idea was to use the Tuxedo workflow (Bowtie + Tophat + Cufflinks) to map the reads back to the genome, reconstruct transcripts, and do differential expression analysis. Unfortunately the latest version of Cufflinks (as of Feb 3 2015) fails for this data set, crashing with a segfault and without any helpful error message.
提供机构:
figshare
创建时间:
2015-02-03
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