Acinetobacter piscicola Genome sequencing. Acinetobacter piscicola

Skatole (3-methylindole, C9H9N) is an odor that is highly offensive, and there is evidence suggesting that skatole poses significant risks to the health of humans and animals. Biodegradation is a feasible method for the removal of skatole, yet the functional genes responsible for the biodegradation of skatole remain largely uncharted territory. Therefore, an in-depth study of the metabolic pathways of skatole is of significant importance for understanding its transformation mechanisms in the environment and for developing effective removal technologies. By revealing the key enzymes and reaction steps in the metabolic pathways, we can provide more effective strategies and methods for environmental protection and pollution control. In this research, we meticulously isolated and purified a bacterial strain, Acinetobacter_piscicola p38, from the fecal samples of pigs, chickens, cattle, and sheep. We undertook an extensive examination of this strain's attributes, particularly its skatole degradation properties, the metabolic products produced by Acinetobacter_piscicola p38 as it metabolizes skatole, a detailed analysis of the strain's complete genome, and the influence of skatole on the gene expression patterns of Acinetobacter_piscicola p38. The Acinetobacter_piscicola p38 strain demonstrated superior degradation capabilities and could completely remove 100 mg/L of skatole in an inorganic salt medium within 6 h. It retained robust degradation performance under the condition of pH value 6.0 - 8.0 and temperature 30 - 40. During the skatole biodegradation process, four significant intermediates were identified: indole, 2-aminoacetophenone, o-aminobenzoic acid, and benzoic acid. The Acinetobacter_piscicola p38 strain utilized skatole as its exclusive carbon and energy source.