Gene expression of metastatic cancer stem cells and non-metastatic cancer stem cells

We have previously isolated CSCs from SKOV-3 and HEYA8 (Chau et al., 2013). Using in vivo selection by orthotopic implantation of CSCs into the bursa of the mouse ovary, we further isolated the highly metastatic population of CSCs (M-CSCs) in SKOV-3, which consistently metastasized to the peritoneum in mice. Conversely, non-metastatic (NM)-CSCs, which are equally tumorigenic, did not metastasize. To identify differentially expressed genes, we profiled gene expression level of M-CSCs and NM-CSCs.