Altering the redox status of Chlamydia trachomatis directly impacts its developmental cycles progression

RNAseq analysis was performed for ahpc Knockdown strain of Chlamydia trachomatis in the unindcued (-aTc) or induced (+aTc) at 14 hpi to monitor transcriptional changes. The transformants of ahpC KD were infected into HeLa cells. At 10 hpi, the constructs were induced or not with 1 nM aTc. RNA samples were prepared from ahpC-KD-infected cells at 14 hpi. 20 μg RNA samples were treated with DNase to remove DNA contamination by using DNA-free Turbo kit (Thermo) according to the manusfacturer’s instruction. Ribosomal RNA was depleted from samples using the MICROBEnrich (Thermo) and MICROBExpress kits (Thermo) following the manufacturer’s instructions. RNA samples were processed for sequencing by the UNMC Genomics Core Facility.