Processed Lipidomics data: Lung adenocarcinoma vs. normal lung tissue (human)

For lipidomic analyses, 22 normal lung tissues and 29 tumor tissues from adenocarcinoma patients undergoing lung resection were analyzed.
Samples were processed as detailed in Hoppstädter et al. (2021, DOI: https://doi.org/10.1016/j.ebiom.2021.103578). The analysis of lipids was performed by direct flow injection analysis (FIA) using a triple quadrupole mass spectrometer (FIA-MS/MS; QQQ triple quadrupole; Micromass Quattro Ultima, Waters) and a hybrid quadrupole-Orbitrap mass spectrometer (FIA-FTMS; high mass resolution; Qexactive Orbitrap, ThermoFisher Scientific).
FIA-MS/MS (QQQ) was performed in positive ion mode using the analytical setup and strategy described previously (Liebisch et al., 2006, doi:10.1016/j.bbalip.2005.12.007; Liebisch et al., 2004, doi: 10.1016/j.bbalip.2004.09.003 ). The Fourier Transform Mass Spectrometry (FIA-FTMS) setup and data processing details are described in (Höring et al., 2019, doi:10.1021/acs.analchem.8b05013).
For PE, PE P, PS, PI, PG, LPC, LPE, SM, Cer, and HexCer, QQQ data were analyzed. For PC, PC O, DG, TG, FC, and CE, FTMS data were used.

在脂质组学分析中，对22例正常肺组织和29例接受肺切除手术的腺癌患者的肿瘤组织进行了分析。样品处理过程详见Hoppstädter等人的研究（2021年，DOI：https://doi.org/10.1016/j.ebiom.2021.103578）。脂质的分析通过直接流动注射分析（FIA）使用三重四极杆质谱仪（FIA-MS/MS；QQQ三重四极杆；Micromass Quattro Ultima，Waters）和混合四极杆-轨道阱质谱仪（FIA-FTMS；高质量分辨率；Qexactive Orbitrap，ThermoFisher Scientific）进行。

FIA-MS/MS（QQQ）在正离子模式下进行，采用先前描述的分析装置和策略（Liebisch等人，2006年，doi：10.1016/j.bbalip.2005.12.007；Liebisch等人，2004年，doi：10.1016/j.bbalip.2004.09.003）。傅里叶变换质谱法（FIA-FTMS）的装置和数据处理细节在（Höring等人，2019年，doi：10.1021/acs.analchem.8b05013）中有所描述。

对于PE、PE P、PS、PI、PG、LPC、LPE、SM、Cer和HexCer，分析了QQQ数据。对于PC、PC O、DG、TG、FC和CE，则使用了FTMS数据。