Transcription profiling of liver from farnesoid X receptor knockout mice

Expression profiling of whole body (WB) FXR knockout (KO) mice (FXR WB KO), liver-specific FXR KO mice (AFXR Cre+) and enterocyte specific FXR KO mice (VFXR Cre+) on a C57BL/6J genetic background Whole body (WB) FXR knockout (KO) mice (FXR WB KO), liver-specific FXR KO mice (AFXR Cre+) and enterocyte specific FXR KO mice (VFXR Cre+) on a C57BL/6J genetic background were bred and maintained in the laboratory animal research facility at the University of Kansas Medical Center in rooms under a 12-hour light-dark cycle. All experiments used 10-16 week-old male mice. FXR was activated by treatment with the FXR synthetic agonist, GW4064, at 150 mg/kg. GW4064 or vehicle was administered by oral gavage at 6 p.m., followed by a second administration at 8 a.m. the next morning. Two hours later, the liver was harvested.本数据集针对C57BL/6J遗传背景下的全身（whole body, WB）FXR敲除（knockout, KO）小鼠（FXR WB KO）、肝脏特异性FXR敲除小鼠（AFXR Cre+）以及肠上皮细胞特异性FXR敲除小鼠（VFXR Cre+）开展表达谱分析。上述三类C57BL/6J遗传背景的小鼠均繁育饲养于堪萨斯大学医学中心实验动物研究设施中，饲养环境为12小时明暗交替循环的饲养室。所有实验均采用10~16周龄的雄性小鼠。采用150 mg/kg的FXR合成激动剂GW4064进行处理以激活FXR：于当日下午6点通过口饲管饲法给予GW4064或溶剂对照，次日上午8点进行第二次给药；给药两小时后，采集肝脏组织。