Next Generation Sequencing measuring Nod-like receptors mRNA expression in intestinal epithelial cells

Intestinal epithelisal cells were obtained by EDTA isolation of ileum from WT mice followed by facs sorting (EpCAM + CD45 – 7AAD – ). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat , and gene expression levels were measured by Cufflinks . Ileum epithelisal cells mRNA profiles were generated by deep sequencing, in duplicate, using illumina HiSeq 2000.